Genome Editing Shared Resource

Overview of Services

The Genome Editing Shared Resource of Rutgers-CINJ (GESR) is a state-of-the-art university core facility that offers services related to the creation of mouse and cell line models for the Rutgers research community. GESR utilizes the latest in genome editing technologies, particularly CRISPR-Cas9, to make precise genetically modified animals and custom modified cell lines tailored to the needs of our investigators.

In addition to gene editing, GESR offers traditional services such as DNA/BAC microinjection to make transgenic mouse lines. The GESR can assist investigators in banking their lines by sperm or embryo cryopreservation.                                                                                                          

GESR utilizes in vitro fertilization (IVF) and embryo rederivation to facilitate import of lines from external sources or from rooms of lower health status into clean facilities. IVF may also be used to quickly expand colonies.  

Rutgers GESR offers the following services:

  • Mouse model generation
  • Cell line gene editing
  • Mouse line cryopreservation
  • Mouse line rederivation
  • Design and production of CRISPR vectors
  • PCR Genotyping
  • Founder breeding

GFP embryo

Mouse embryos expressing Rosa26-targeted human ACE2 and GFP after Cre-mediated removal of the loxP-STOP-loxP cassette in vivo.

This constitutively expressing human ACE2 mouse model is one of several made by GESR to study COVID-19.

The GESR can also work with you on special projects to study embryonic development, in vivo gene editing/therapeutic models and control of gene expression using CRISPR-based activators/repressors that go beyond gene editing.

 

WHAT'S NEW and AVAILABLE from Rutgers Genome Editing Shared Resource in 2020?

NEW-Efficient targeting at H11 locus (a safe harbor locus that is an alternative to Rosa26). Expand your repertoire of targeted lines with new reporters expressed from the H11 locus.

Also, instead of making traditional transgenics with variable expression, knock it into H11 instead to get reliable expression and screen fewer lines.

Fluorescent microscope image of an ear punch from a single copy Cag-mCherry transgene knocked-into the H11 locus using CRISPR-Cas9 

Fluorescent microscope image of an ear punch from a mouse with a single copy

Cag-mCherry transgene knocked-into the mouse H11 (safe harbor) locus using CRISPR-Cas9

 

NEW-Direct knock-in into NSG background (or any strain background, just inquire)

 

NEW- Ask us how you can KO up to 10 genes simultaneously in vivo in a tissue-specific manner using our own experimental conditional Cre-mediated Cas9 expression system.

 

 

 

Leadership

Peter Romanienko, PhD Managing Director 7322355978 pjr110@cinj.rutgers.edu

Location and hours of operation

Hours Location

 Monday - Friday 
 9AM - 5PM 

 89 French Street 
 New Brunswick, NJ 08901 

 

 

 

Contacts

Name Role Phone Email Location
Peter Romanienko, PhD
Managing Director
 
7322355978
 
pjr110@cinj.rutgers.edu
 

 

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